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| 7. |  | MAZZONI, R.; MORAES, M.; REZENDE, C. F.; MIRANDA, J. C. Alimentação e padrões ecomorfológicos das espécies de peixes de riacho do alto rio Tocantins, Goiás, Brasil. Iheringia: Série Zoologica, Porto Alegre, v. 100, n. 2, p. 162-168, 2010.| Biblioteca(s): Epagri-Sede. |
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| 14. | | ARAUJO, L.; CARDOZA, Y. F.; DUARTE, V.; MORAES, M. G. Pseudomonas syringae causing bacterial canker on apple trees in Brazil. Bragantia, Campinas, SP, v. 79, n. 4, p. 1-7, 2020.| Biblioteca(s): Epagri-Sede. |
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| 16. | | ARAUJO, L.; CARDOZA, Y. F.; DUARTE, V.; MORAES, M. G. Symptoms of bacterial shoot blight associated with Pseudomonas syringae pv. syringae in apple trees in Brazil. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 51., 2019, Recife, PE. Resumos... Recife, PE: Sociedade Brasileira de Fitopatologia, 2019. p. 92| Biblioteca(s): Epagri-Sede. |
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| 19. | | CANUTO, D.S. de O.; SILVA, A.M. da; MORAES, M.A. de; SILVA, C.L.S.P. da; MORAES, M.L.T. de; SÁ, M.E. de Variabilidade genética de populações naturais de Dipteryx alata Vog. por meio de caracteres nutricionais em sementes. Revista do Instituto Florestal, São Paulo, SP, v. 20, n.2, p.155-163, dez. 2008.| Biblioteca(s): Epagri-Sede. |
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Registro Completo
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Biblioteca(s): |
Epagri-Sede. |
|
Data corrente: |
09/11/2020 |
|
Data da última atualização: |
09/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Internacional - B |
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Autoria: |
ARAUJO, L.; CARDOZA, Y. F.; DUARTE, V.; MORAES, M. G. |
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Título: |
Pectobacterium carotovorum subsp. actinidiae associated with canker on pear trees in Brazil. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
European Journal of Plant Pathology, Holanda, v. 158, n. 4, p. 1-8, 2020. |
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Idioma: |
Inglês |
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Conteúdo: |
In summer of 2016, cankers were observed
on 8-year-old pear trees (Pyrus communis L.) cv. Yali
grafted on the Caleriana rootstock, in a commercial
orchard in Monte Castelo, SC. Eight hundred out of
1200 trees died and the rest of the orchard was eradicated.
Affected plants showed cankers on trunks and twigs
with exudation; the branches turned brown and slowly
died. Bacterial colonies were isolated on King?s B medium
from infected tissues. Pathogenicity, biochemical
tests were conducted and some isolates selected for
whole genome sequencing using Ion Personal Genome
Machine? (PGM?) System (ThermoFisher Scientific).
PCR with Y1/Y2 and PcaF/PcaR primers, specific
for Pectobacterium carotovorum and Pectobacterium
carotovorum subsp. actinidiae, respectively, were also
carried out. The section of inoculated apple and pear
branches showed longitudinal darkening, with wilt and
leaf blight. Shoots of kiwifruit Actinidia deliciosa cv.
Hayward inoculated with the bacterium showed no
symptoms. The bacterium was re-isolated from all
symptomatic tissues, satisfying Koch?s postulates. Selected
isolates were gram-negative, facultative anaerobic,
rod-shaped and exhibited a strong pectolytic activity
in potato and carrot disks, and showed similar biochemical
profile using a BIOLOG GEN III MicroPlate. The
pathogen of the symptoms observed on pear trees in
Brazil show highest genome (87.1%; DDH analysis)
and proteome identity (99.8%; Patric analysis) with
P. carotovorum subsp. actinidiae. A product of the
expected size was obtained in both specific PCR (Y1/
Y2 (434 bp) and PcaF/PcaR (343 bp) primer pairs).
Results from the present study clearly show that P.
carotovorum subsp. actinidiae was associated with canker
on pear trees in Santa Catarina, Brazil. MenosIn summer of 2016, cankers were observed
on 8-year-old pear trees (Pyrus communis L.) cv. Yali
grafted on the Caleriana rootstock, in a commercial
orchard in Monte Castelo, SC. Eight hundred out of
1200 trees died and the rest of the orchard was eradicated.
Affected plants showed cankers on trunks and twigs
with exudation; the branches turned brown and slowly
died. Bacterial colonies were isolated on King?s B medium
from infected tissues. Pathogenicity, biochemical
tests were conducted and some isolates selected for
whole genome sequencing using Ion Personal Genome
Machine? (PGM?) System (ThermoFisher Scientific).
PCR with Y1/Y2 and PcaF/PcaR primers, specific
for Pectobacterium carotovorum and Pectobacterium
carotovorum subsp. actinidiae, respectively, were also
carried out. The section of inoculated apple and pear
branches showed longitudinal darkening, with wilt and
leaf blight. Shoots of kiwifruit Actinidia deliciosa cv.
Hayward inoculated with the bacterium showed no
symptoms. The bacterium was re-isolated from all
symptomatic tissues, satisfying Koch?s postulates. Selected
isolates were gram-negative, facultative anaerobic,
rod-shaped and exhibited a strong pectolytic activity
in potato and carrot disks, and showed similar biochemical
profile using a BIOLOG GEN III MicroPlate. The
pathogen of the symptoms observed on pear trees in
Brazil show highest genome (87.1%; DDH analysis)
and proteome identity (99.8%; Patric analysis) with
P. carotovorum subsp. actinidiae. A pro... Mostrar Tudo |
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Palavras-Chave: |
Actinidia chinensis; Actinidia deliciosa; Bacterial diseases; Malus domestica; Pyrus communis; Wilting. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02454naa a2200229 a 4500
001 1130130
005 2020-11-09
008 2020 bl uuuu u00u1 u #d
100 1 $aARAUJO, L.
245 $aPectobacterium carotovorum subsp. actinidiae associated with canker on pear trees in Brazil.$h[electronic resource]
260 $c2020
520 $aIn summer of 2016, cankers were observed on 8-year-old pear trees (Pyrus communis L.) cv. Yali grafted on the Caleriana rootstock, in a commercial orchard in Monte Castelo, SC. Eight hundred out of 1200 trees died and the rest of the orchard was eradicated. Affected plants showed cankers on trunks and twigs with exudation; the branches turned brown and slowly died. Bacterial colonies were isolated on King?s B medium from infected tissues. Pathogenicity, biochemical tests were conducted and some isolates selected for whole genome sequencing using Ion Personal Genome Machine? (PGM?) System (ThermoFisher Scientific). PCR with Y1/Y2 and PcaF/PcaR primers, specific for Pectobacterium carotovorum and Pectobacterium carotovorum subsp. actinidiae, respectively, were also carried out. The section of inoculated apple and pear branches showed longitudinal darkening, with wilt and leaf blight. Shoots of kiwifruit Actinidia deliciosa cv. Hayward inoculated with the bacterium showed no symptoms. The bacterium was re-isolated from all symptomatic tissues, satisfying Koch?s postulates. Selected isolates were gram-negative, facultative anaerobic, rod-shaped and exhibited a strong pectolytic activity in potato and carrot disks, and showed similar biochemical profile using a BIOLOG GEN III MicroPlate. The pathogen of the symptoms observed on pear trees in Brazil show highest genome (87.1%; DDH analysis) and proteome identity (99.8%; Patric analysis) with P. carotovorum subsp. actinidiae. A product of the expected size was obtained in both specific PCR (Y1/ Y2 (434 bp) and PcaF/PcaR (343 bp) primer pairs). Results from the present study clearly show that P. carotovorum subsp. actinidiae was associated with canker on pear trees in Santa Catarina, Brazil.
653 $aActinidia chinensis
653 $aActinidia deliciosa
653 $aBacterial diseases
653 $aMalus domestica
653 $aPyrus communis
653 $aWilting
700 1 $aCARDOZA, Y. F.
700 1 $aDUARTE, V.
700 1 $aMORAES, M. G.
773 $tEuropean Journal of Plant Pathology, Holanda$gv. 158, n. 4, p. 1-8, 2020.
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